Investigation

Research Interest:

Molecular biology of filamentous fungi in relation to penicillin and cephalosporinbiosynthesis. Fungal genoma and chromosome analysis.
Optimization of the mechanisms leading to overexpression and secretionof heterologous proteins in fungi.
Biosynthesis and Molecular genetics of pigments production by Monascussp

Cloning of genes involved in beta-lactam antibiotic biosynthesis: CephamycinC and clavulanic acid.

Phosphate and iron regulation of gene expression.

Biosynthesis and genetics of polyene macrolides production.

Genome analysis

SCIENTIFIC PUBLICATIONS AFTER 1997.

1997

Pérez-Llarena FJ, Liras P., García-Rodriguez A. & Martín JF. A regulatory gene (ccaR) required for cephamycin and clavulanic acid production in Streptomyces clavuligerus:Amplification results in overproduction of both beta-lactam compounds.J. Bacteriol. 179, 2053-2059.

Romero J, Martín JF & Liras P, Demain AL &Rius N. Partial purification, chracterization and nitrogen regulation of the lysine 6-aminotransferase of Streptomyces clavuligerus. J. Ind.Microbiol. Biotechnol. 18, 241-246.

Oguiza JA , Marcos AT, & Martín JF. Transcriptional analysis of the sigA and sigB genes of Brevibacterium lactofermentum. FEMS Microbiol. Lett.153, 111-117.

Blanco J, Coque JJR, Velasco J, & MartínJF. Cloning, expression in Streptomyces lividans and biochemical characterization of a thermostable endo-ß-1,4-xylanase. Appl. Microbiol.Biotechnol. 48; 208-217.

Leitão AL, Enguita FJ, & Martín JF.Improved oxygen transfer in cultures of Nocardia lactamdurans maintains the cephamycin biosynthetic proteins for prolonged time and enhances the conversion of deacetylcephalosporin into cephamycin C. J. Biotechnol. 58,39-50.

Hijarrubia MJ, Casqueiro J, Gutiérrez S, FernándezFJ & Martín J.F. Characterization of the bip gene of Aspergillus awamori encoding a protein with a HDEL retention signal homologous to the mammalian BiP involved in polypeptide secretion. Curr Genet.32, 139-146.

Fuente JL, Rumbero A., Martín JF & Liras P.1-Piperideine-6-Carboxylate dehydrogenase, a new enzyme that forms alpha-aminoadipate in Streptomyces clavuligerus and other cephamycin C-producing actinomycetes. Biochem.J. 327, 59-64.

Gutiérrez S, Velasco J, Marcos AT, FernándezFJ, Fierro F, Barredo JL, Díez B & Martín J.F. Expression of the cefG gene is limiting for cephalosporin biosynthesis in Acremoniumchrysogenum. Appl. Microbiol. Biotechnol. 48, 606-614.

Martín JF., Gutiérrez S., Demain A.L. ß-lactamsin "Fungal Biotechnology", Ed. by T. Anke, Chapman & Hall. Weinheim.

Gil, J.A. & Martín, J.F. Polyene Antibiotics.In “Biotechnology of Antibiotics”, pp. 551-576. Vol.19 (Strohl WR, ed).Marcel Dekker. New York.

Rodríguez-Garcia, A., Ludovice, M., Martín,J.F. and Liras, P. Arginine boxes and the argR gene in Streptomyces clavuligerus: evidence for a clear regulation of the arginine pathway.Mol. Microbiol. 25, 219-228.

Chary V.K., Fuente, J.L., Liras, P. & Martín,J.F. amy as a reporter gene for promoter activity in Nocardia lactamdurans: Comparison of promoters of the cephamycin cluster. Appl.Env. Microbiol. 63, 2977-2982

Pérez-Llarena, F.J., Martín, J.F., Galleni,M., Coque, J.R., Fuente, J.L., Frere, J.M. and Liras, P. The bla gene of the cephamycin cluster of Streptomyces clavuligerus encodes a class A b-lactamase of low enzymatic activity.J. Bacteriol. 179, 6035-6040.

1998

Blanco J,Rubio Coque J.J., Martín J.F. The Folate Branch of the Methionine Biosynthesis Pathway in Streptomyces lividans:Disruption of the 5,10-Methylenetrahydrofolate Reductase Gene Leads to Methionine Auxotrophy. J. Bacteriol. 180, 6, 1586-1591

Pisabarro A., Correia A & Martín JF.Characterizationof the rrnB Operon of the Plant Pathogen Rhodococcus fasciansand Targeted Integrations of Exogenus Genes at rrn Loci. Appl. Env.Microbiol. 64. 4, 1276-1282

Pisabarro A., Correia A.& Martín JF.Pulsed-Field Gel Electrophoresis Analysis of the genome of Rhodococcusfascians: Genome Size and Linear and Circular Replicon Coimpositionin Virulent and Avirulent Strains.Current Microbiol. 36, 302-308

Martín, J.F. New aspects and enzymes for b-lactambiosynthesis. Appl. Microbiol. Biotechnol. 50, 1-15.

Pérez-Llarena, F.J., Rodríguez-Garcia, A.,Enguita, F.J., Martín, J.F. & Liras, P. The pcd gene encodingpiperideine-6-carboxylate dehydrogenase involved in biosynthesis of a-aminoadipic acid is located in the cephamycincluster of Streptomyces clavuligerus. J. Bacteriol. 180, 4753-4756.

Cardoza, R.E., Moralejo, F., Gutierrez, S., Casqueiro,J., Fierro, F., & Martín, J.F. Characterization and nitrogensource regulation at the transcript level of the gdhA gene of Aspergillusawamori, encoding an NADP-dependent glutamate dehydrogenase. C. Genet.34, 50-59.

Pérez-Redondo, R., Rodriguez-Garcia, A., MartínJ.F. and Liras, P. The claR gene of Streptomyces clavuligerus,encoding a lysR type regulatory protein controlling clavulanic acidbiosynthesis is linked to the clavulanate-9-aldehyde reductase (car)gene. Gene 211, 311-321.

Casqueiro, J., Gutiérrez, S., Bañuelos, O., Fierro, F., Velasco, J. y Martín, J.F. "Characterization of the lys2 gene of Penicillium chrysogenum encoding a-aminoadipic acid reductase". Mol. Gen. Genet. 259:549-556

Enguita, F.J., Coque, J.J.R., Liras, P. and Martín, J.F. "The nine genes of the Nocardia lactamdurans cephamycin cluster are transcribed into large mRNAs from three promoters, two of them located in a bidirectional promoter region". J. Bacteriol. 180:5489-5494

Blanco, J., Driessen, J.M., Coque, J.J.R. and Martín, J.F. "Biochemical characterization of the SecA protein of Streptomyces lividans. Interaction with nucleotides, binding to membrane vesicles and in vitro translocation of proAmy protein". Eur. J. Biochem. 257:472-478

Liras, P., Rodríguez-García, A. y Martín, J.F. "Evolution of the clusters of genes for ß-lactam antibiotics: a model for evolutive cominatorial assembling of new ß-lactams". Internatl. Microbiol. 1:271-278

Martín, J.F. and Gutiérrez, S., Eds. (1998) "Fungal Genetics". Servicio de Publicaciones Universidad de León, pp. 321. ISBN 84-7719-673-7.

Demain, A.L., Martín, J.F. and Elander, R.P. (1998) "Penicillin biochemistry and genetics". In: Penicillin, a paradigm for biotechnology. Mateles, R.I. (ed.). Candida, Co., Chicago, USA, pp. 93-113.

Martín, J.F. and Gutiérrez, S., Eds. (1998) "Fungal Genetics". Servicio de Publicaciones Universidad de León, pp. 321. ISBN 84-7719-673-7.

Demain, A.L., Martín, J.F. and Elander, R.P. (1998) "Penicillin biochemistry and genetics". In: Penicillin, a paradigm for biotechnology. Mateles, R.I. (ed.). Candida, Co., Chicago, USA, pp. 93-113.

1999

Velasco, J, Gutiérrez, S., Campoy, S. and Martín, J.F. "Molecular characterization of the Acremonium chrysogenum cefG gene product: the native deacetylcephalosporin C acetyltransferase is not processed into subunits". Biochem. J. 337:379-385

Gutiérrez, S., Marcos, A.T., Casqueiro, J., Kosalková, K., Fernández, F.J., Velasco, J. y Martín, J.F. "Transcription of the pcbAB, pcbC and penDE genes of Penicillium chrysogenum AS-P-78 is repressed by glucose and the repression is not reversed by alkaline pHs". Microbiology 145:317-324

Bañuelos, O., Casqueiro, J., Fierro, F., Hijarrubia, M.-J., Gutiérrez, S. and Martín, J.F. "Characterization and lysine control of expression of the lys1 gene of Penicillium chrysogenum encoding homocitrate synthase". Gene 226:51-59

Fierro, F. and Martín, J.F. "Molecular mechanism of chromosomal rearrangement in fungi". Crit. Rev. Microbiol. 25(1):1-17

Casqueiro, J., Gutiérrez, S., Bañuelos, O., Hijarrubia, M.J. and Martín, J.F. "Gene targeting in Penicillium chrysogenum: Disruption of the lys2 gene leads to penicillin overproduction". J. Bacteriol. 181:1181-1188

Martín, J.F. and Gil, J.A. "Corynebacteria". In: Demain, A.L., Davies, J.E., Atlas, R.M., Cohen, G., Hershberger, C.L., Hu, W.-S., Sherman, D.H., Willson, R.C. and Wu, J.H.D. (eds.) Manual of Industrial Microbiology and Biotechnology, 2nd Ed., pp. 379-391. ASM Press, Washington, D.C.

Laich, F., Fierro, F., Cardoza, R.E. and Martín, J.F. "Organization of the gene cluster for biosynthesis of penicillin in Penicillium nalgiovense and antibiotic production in cured dry sausages". Appl. Environm. Microbiol. 65:1236-1240

Moralejo, F.J., Cardoza, R.E., Gutiérrez, S. and Martín, J.F. "Thaumatin production in Aspergillus awamori by use of expression cassettes with strong fungal promoters and high gene dosage". Appl. Environm. Microbiol. 65:1168-1174

Amador, E., Castro, J.M., Correia, A. and Martín, J.F. "Structure and organization of the rrnD operon of Brevibacterium lactofermentum: analysis of the 16s rRNA gene". Microbiology 145:915-924

Aparicio, J.F., Colina, A.J., Ceballos, E. and Martín, J.F. "The biosynthetic gene cluster for the 26-membered ring polyene macrolide pimaricin: A new polyketide synthase organization encoded by two subclusters separated by functionalization genes". J. Biol. Chem. 274:10133-10139

Leitao, A.L., Enguita, F.J., de la Fuente, J.L., Liras, P. and Martín, J.F. "Inducing effect of diamines on transcription of the cephamycin C genes from the lat and pcbAB promoters in Nocardia lactamdurans". J. Bacteriol. 181:2379-2384

Martín, J.F., Casqueiro, J., Kosalková, K., Marcos, A.T. and Gutiérrez, S. "Penicillin and cephalosporin biosynthesis: Mechanism of carbon catabolite regulation of penicillin production". Antonie van Leeuwenhoek 75:21-31

Gutiérrez, S., Fierro, F., Casqueiro, J. and Martín, J.F. "Gene organization and plasticity of the ß-lactam genes in different filamentous fungi". Antonie van Leewenhoek 75:81-94

Liras, P. "Biosynthesis and molecular genetics of cephamycins". Antonie van Leewenhoek 75:109-124

Velasco, J, Gutiérrez, S., Campoy, S. and Martín, J.F. "Molecular characterization of the Acremonium chrysogenum cefG gene product: the native deacetylcephalosporin C acetyltransferase is not processed into subunits". Biochem. J. 337 :379-385

Gutiérrez, S., Marcos, A.T., Casqueiro, J., Kosalková, K., Fernández, F.J., Velasco, J. and Martín, J.F. "Transcription of the pcbAB, pcbC and penDE genes of Penicillium chrysogenum AS-P-78 is repressed by glucose and the repression is not reversed by alkaline pHs". Microbiology 145 :317-324

Fierro, F. and Martín, J.F. "Molecular mechanism of chromosomal rearrangement in fungi". Crit. Rev. Microbiol. 25 (1):1-17

Amador, E., Castro, J.M., Correia, A. and Martín, J.F. "Structure and organization of the rrnD operon of Brevibacterium lactofermentum: analysis of the 16s rRNA gene". Microbiology 145 :915-924

Leitao, A.L., Enguita, F.J., de la Fuente, J.L., Liras, P. and Martín, J.F. "Inducing effect of diamines on transcription of the cephamycin C genes from the lat and pcbAB promoters in Nocardia lactamdurans". J. Bacteriol. 181 :2379-2384

Gutiérrez, S., Fierro, F., Casqueiro, J. and Martín, J.F. "Gene organization and plasticity of the ß-lactam genes in different filamentous fungi". Antonie van Leewenhoek 75 :81-94

Casqueiro, J., Bañuelos, O., Gutiérrez, S., Hijarrubia, M.J. and Martín, J.F. "Intrachromosomal recombination between direct repeats in Penicillium chrysogenum: gene conversion and deletion events". Mol. Gen. Genet. 261 :994-1000

Pérez-Redondo, R., Rodríguez-García, A., Martín, J.F. and Liras, P. "Deletion of the pyc gene blocks clavulanic acid biosynthesis except in glycerol-containing medium: Evidence for two different genes in formation of the C3 unit". J. Bacteriol. 181 :6922-6928

Sandoval H, Manga-Gonzalez Y, Campo R, Garcia P, Castro JM, Perez de la Vega M Preliminary study on genetic variability of Dicrocoelium dendriticum determined by random amplified polymorphic DNA.Parasitol Int. 1999 Mar;48(1):21-6.

2000

Liras, P. y Rodríguez-García, A.Clavulanic acid, a b-lactamase inhibitor: Biosynthesis and molecular genetics. Applied Microbiology and Biotechnology , 54, 467-475. 2000

Bañuelos, O., Casqueiro, J., Gutiérrez, S., Riaño, J. and Martín, J.F. "The specific transport system for lysine is fully inhibited by ammonium in Penicillium chrysogenum: an ammonium-insensitive system allows uptake in carbon-starved cells". Antonie van Leeuwenhoek 77:91-100

Mendes, M.V., Aparicio, J.F. and Martín, J.F. “Complete nucleotide sequence and characterization of pSNA1 from pimaricin-producing Streptomyces natalensis that replicates by a rolling circle mechanism. Plasmid 43:159-165

Kosalková, K., Marcos, A.T., Fierro, F., Hernando-Rico, V, Gutiérrez, S. and Martín, J.F. "A novel heptameric sequence (TTAGTAA) is the binding site for a protein required for high level expression of pcbAB, the first gene of the penicillin biosynthesis in Penicillium chrysogenum". J. Biol. Chem. 275:2423-2430

Barreiro, C., Pisabarro, A. and Martín, J.F. “Characterization of the ribosomal rrnD operon of the cephamycin-producer ‘Nocardia lactamdurans’ shows that this actinomycete belongs to the genus Amycolatopsis”. System. Appl. Microbiol. 23:15-24

Chary, V.K., de la Fuente, J.L., Leitão, A.L., Liras, P. and Martín, J.F. "Overexpression of the lat gene in Nocardia lactamdurans from strong heterologous promoters results in very high levels of lysine-6-aminotransferase and up to two-fold increase in cephamycin C production". Appl. Microbiol. Biotechnol. 53 :282-288

Martín, J.F. “Molecular control of expression of penicillin biosynthesis genes in fungi: Regulatory proteins interact with a bidirectional promoter region”. J. Bacteriol. 182 :2355-2362

Amador, E., Martín, J.F. and Castro, J.M. “A Brevibacterium lactofermentum 16S rRNA gene used as target site for homologous recombination”. FEMS Microbiol. Lett. 185 :199-204

Gutiérrez, S., Casqueiro, J. y Martín, J.F. “Los hongos como factorías celulares: biodiversidad de metabolitos secundarios”. Rev. Iberoam. Micol. 17:S54-S60

Bañuelos, O., Casqueiro, J., Gutiérrez, S. and Martín, J.F. “Overexpression of the lys1 gene in Penicillium chrysogenum: Homocitrate synthase levels, a-aminoadipic acid pool and penicillin production. Appl. Microbiol. Biotechnol. 54 :69-77

Cardoza, R.E., Velasco, J., Martín, J.F. and Liras, P. “A cephalosporin C acetylhydrolase is present in the cultures of Nocardia lactamdurans”. Appl. Microbiol. Biotechnol. 54 :406-412

Martín, J.F., Gutiérrez, S. and Aparicio, J.F. "Secondary metabolites". En: "Encyclopedia of Microbiology", vol. 4, 2nd ed. (Lederberg, J., ed.). Academic Press. San Diego, CA, pp. 213-237.

Rodríguez-García, A., de la Fuente, A., Pérez-Redondo, Martín, J.F. and Liras, P. “Characterization and expression of the arginine biosynthesis gene cluster of Streptomyces clavuligerus”. J. Mol. Microbiol. Biotechnol. 2 :543-550

Martín, J.F. “a-aminoadipyl-cysteinyl-valine synthetases in b-lactam producing organisms. From Abraham’s discoveries to novel concepts of non-ribosomal peptide synthesis”. J. Antibiot. 53:1008-1021

Fierro, F., Gutiérrez, S. and Martín, J.F. “Genes for b-lactam antibiotics: clustering and amplification”. En: Recent Research Developments in Microbiology. Pandalai, S.G. (ed.). Research Sign Post.

Aparicio, J.F., Fouces, R., Mendes, M.V., Olivera, N. and Martín, J.F. “A complex multienzyme system encoded by five polyketide synthase genes is involved in the biosynthesis of the 26-membered polyene macrolide pimaricin in Streptomyces natalensis”. Chemistry & Biology 7 :895-905

Moralejo, F.J., Cardoza, R.E., Gutiérrez, S., Sisniega, H., Faus, I. and Martín, J.F. “Overexpression and lack of degradation of thaumatin in an aspergillopepsin-A defective mutant of Aspergillus awamori containing an insertion in the pepA gene”. Appl. Microbiol. Biotechnol. 54 :772-777

2001

Casqueiro, J., Bañuelos, O., Gutiérrez, S. and Martín, J.F. “Metabolic engineering of the lysine pathway for b-lactam overproduction in Penicillium chrysogenum”. En: Novel Frontiers in the Production of Compounds for Biomedical Use. Focus on Biotechnology, vol. 1. Van Broedkhoven, A., Anné J. and Shapiro, F (eds.). Kluwer Academic Publishers, NL, pp. 147-159

Liu, G., Casqueiro, J., Bañuelos, O., Cardoza, R.E., Gutiérrez, S. and Martín, J.F. “Targeted inactivation of the mecB gene encoding cystathionine-g-lyase shows that the transsulfuration pathway is required for high level cephalosporin biosynthesis in Acremonium chrysogenum C10 but not for methionine-induction of the cephalosporin genes”. J. Bacteriol. 183 :1765-1772

Marcos, A.T., Kosalková, K., Cardoza, R.E., Fierro, F., Gutiérrez, S. and Martín, J.F. “Characterization of the reverse transsulfuration mecB gene of Acremonium chrysogenum encoding a functional cystathionine-g-lyase. Mol. Gen. Genet. 264 :746-754

Hijarrubia, M.J., Aparicio, J.F. Casqueiro, J. and Martín, J.F. “Characterization of the lys2 gene of Acremonium chrysogenum encoding a functional a-aminoadipate activating and reducing enzyme”. Mol. Gen. Genet. 264 :755-762

Barreiro, C., González-Lavado, E. and Martín, J.F. “Organization and transcriptional analysis of a six gene cluster around the rplK-rplA operon of Corynebacterium glutamicum encoding the ribosomal proteins L11 and L1. J. Bacteriol. 67 :2183-2190

Wehmeier, L., Brockmann-Gretza, O., Pisabarro, A., Tauch, A., Pühler, A., Martín, J.F. and Kalinowski, J. “A Corynebacterium glutamicum mutant with a defined deletion within the rplK gene is impaired in (p)ppGpp accumulation upon amino acid starvation. Microbiology 147 :691-700

Moura, R.S., Martín, J.F., Martín, A. and Liras, P. “Substrate analysis and molecular cloning of the extracellular alkaline phosphatase of Streptomyces griseus”. Microbiology 147 :1525-1533

Hernando-Rico, V., Martín, J.F., Santamarta, I. and Liras, P. “Structure of the ask-asd operon and formation of aspartokinase subunits in the cephamycin producer ‘Amycolatopsis lactamdurans’”. Microbiology 147 :1547-1555

Bañuelos, O., Casqueiro, J., Gutiérrez, S. and Martín, J.F. “Intrachromosomal recombination after targeted monocopy integration in Penicillium chrysogenum: stabilization of the direct repeats to prevent loss of the inserted gene”. Curr. Genet. 39 :231-236

Mendes, M.V., Recio, E., Fouces, R., Luiten, R., Martín, J.F. and Aparicio, J.F. “Engineered biosynthesis of novel polyenes: a pimaricin derivative produced by targeted gene disruption in Streptomyces natalensis”. Chemistry & Biology 8 :635-644

Teves, F.G., Casqueiro, J., Bañuelos, O., Raymundo, A.K. and Martín, J.F. “Elucidation of the regulatory functions of the lys3 gene encoding homoaconitase from Penicillium chrysogenum in the a-aminoadipate pathway”. In: Proceedings of the 4^th Asia-Pacific Biotechnology Congress & 30^th Annual Convention of the Philippine Society for Microbiology, Inc., pp. 252-260

Gang, L., Casqueiro, J., Gutiérrez, S., Kosalková, K., Castillo, N.-I. and Martín, J.F. “Elicitation of penicillin biosynthesis by alginate in Penicillium chrysogenum exerted on pcbAB, pcbC and penDE genes at transcriptional level”. J. Microbiol. Biotechnol. 11 :812-818

Leitao, A.L., Enguita, F.J., Martín, J.F. and Santos Oliveira, J.F. (2001) Effect of exogenous lysine on the expression of early cephamycin C biosynthetic genes and antibiotic production in Nocardia lactamdurans MA4213. Appl. Microbiol. Biotechnol. 56 :670-675

Moralejo, F.J., Watson, A.J., Jeenes, D.J., Archer, D.B. and Martín, J.F. “A defined level of protein disulfide isomerase expression is required for optimal secretion of thaumatin from Aspergillus awamori”. Mol. Gen. Genet. 266 :246-253

Velasco, J., Gutiérrez, S., Casqueiro, J., Fierro, F., Campoy, S. and Martín, J.F. “Cloning and characterization of the gene cahB encoding a cephalosporin C acetylhydrolase from Acremonium chrysogenum”. Appl. Microbiol. Biotechnol. 57 :300-356

Kosalková, K., Marcos, A.T. and Martín, J.F. “A moderate amplification of the mecB gene encoding sytathionine-g-lyase stimulates cephalosporin biosynthesis in Acremonium chrysogenum”. J. Ind. Microbiol. Biotechnol. 27 :252-258

Naranjo, L., Martín de Valmaseda, E., Bañuelos, O., López, P., Riaño, J., Casqueiro, J. and Martín, J.F. “The conversión of pipecolic acid into lysine in Penicillium chrysogenum requires pipecolate oxidase and saccharopine reductase: Characterization of the lys7 gene encoding saccharopine reductase”. J. Bacteriol. 183 :7165-7172

Chávez, R., Fierro, F., Gordillo, F., Martín, J.F. and Eyzaguirre. “Electrophoretic karyotype of the filamentous fungus Penicillium purpurogenum and chromosomal location of several xylanolytic genes”. FEMS Microbiol. Lett. 205 :379-383

Santos, M., Rebordinos, L., Gutiérrez, S., Cardoza, R.E., Martín, J.F. and Cantoral, J.-M. “Characterization of the gdhA gene from the phytopathogen Botrytis cinerea”. Fungal Gen. Biol. 34 :193-206

Chávez, R., Fierro, F., Gordillo, F., Martín, J.F. and Eyzaguirre, J. “Electrophoretic katyorype of the filamentous fungus Penicillium purpurogenum and chromosomal location of several xylanolytic genes”. FEMS Microbiol. Lett. 205 :379-383

Fierro, F., Gutiérrez, S., Casqueiro, J. and Martín, J.F. “Karyotyping of fungi by pulsed field gel electrophoresis”. Recent Res. Devel. Genet. 1 :105-125

Adham SA, Honrubia P, Diaz M, Fernandez-Abalos JM, Santamaria RI, Gil JA Expression of the genes coding for the xylanase Xys1 and the cellulase Cel1 from the straw-decomposing Streptomyces hastedii JM8 cloned into the amino-acid producer Brevibacterium lactofermentum ATCC13869.Arch Microbiol. 2001 Dec;177(1):91-7.

Adham SA, Campelo AB, Ramos A, Gil JA Construction of a xylanase-producing strain of Brevibacterium lactofermentum by stable integration of an engineered xysA gene from Streptomyces halstedii JM8.Appl Environ Microbiol. 2001 Dec;67(12):5425-30.

Honrubia MP, Ramos A, Gil JA The cell division genes ftsQ and ftsZ, but not the three downstream open reading frames YFIH, ORF5 and ORF6, are essential for growth and viability in Brevibacterium lactofermentum ATCC 13869.Mol Genet Genomics. 2001 Aug;265(6):1022-30.

2002

Mellado, E., Lorenzana, L.M., Rodríguez-Sáiz, M., Díez, B, Liras, P. and Barredo, JL. The clavulanic acid biosynthetic cluster of Streptomyces clavuligerus: genetic organization of the region upstream of the car gene.Microbiology Microbiology. 148:1427-38.2002.

Bañuelos, O., Casqueiro, J., Steidl, S., Gutiérrez, S., Brakhage, A. and Martín, J.F. “Subcellular localization of the homocitrate synthase in Penicillium chrysogenum”. Mol. Gen. Genet. 266 :711-719

Laich, F., Fierro, F. and Martín, J.F. “Production of penicillin by fungi growing on food products: Identification of a complete penicillin gene cluster in Penicillium griseofulvum and a truncated cluster in Penicillium verrucosum. Appl. Environ. Microbiol. 68 :1211-1219

Fernández, M., Cuadrado, Y., Recio, E., Aparicio, J.F. and Martín, J.F. “Characterisation of the hom-thrC-thrB cluster in aminoethoxyvinylglycine-producing Streptomyces sp. NRRL 5331”. Microbiology 148 :413-20.

Moralejo, F.J., Cardoza, R.E., Gutiérrez, S., Lombraña, M., Fierro, F. y Martín, J.F. (2002) Silencing of the aspergillopepsin B (pepB) gene of Aspergillus awamori by antisense RNA expression or protease removal by gene disruption results in a high increase of thaumatin production. Appl. Environ. Microbiol. 68 :3550-3559

Santamarta, I., Rodríguez-García, A., Pérez-Redondo, R., Martín, J.F. and Liras, P. “CcaR is an autoregulatory protein that binds to the ccaR and cefD-cmcI promoters of the cephamycin C-clavulanic acid cluster in Streptomyces clavuligerus”. J. Bacteriol. 184 :3106-3113

Hijarrubia, M.J., Aparicio, J.F. and Martín, J.F. “Nitrate regulation of a-aminoadipate reductase formation and lysine inhibition of its activity in Penicillium chrysogenum and Acremonium chrysogenum”. Appl. Microbiol. Biotechnol. 59:270-277

Ullán, R.V., Liu, G., Casqueiro, J., Gutiérrez, S., Bañuelos, O. y Martín, J.F. The cefT gene of Acremonium chrysogenum C10 encodes a putative multidrug efflux pump protein that significantly increases cephalosporin C production. Mol. Gen. Genet. 267:673-683

Casqueiro, J., Bañuelos, O., Gutiérrez, S., Fierro, F. and Martín. J.F. “Site directed integration in b-lactam producing fungi: Molecular mechanisms, genetic instability and industrial application”. Recent Res. Devel. Genet. 2:31-41

Martín, J.F. and Demain, A.L. “Unraveling the methionine-cephalosporin puzzle in Acremonium chrysogenum”. Trends Biotechnol. 20:12502-12507

Ullán, R.V., Casqueiro, J., Bañuelos, O., Fernández, F.J., Gutiérrez, S., and Martín, J.F. “A novel epimerization system in fungal secondary metabolism involved in the conversion of isopenicillin N into penicillin N in Acremonium chrysogenum”. J. Biol. Chem. (en prensa)

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